High-resolution stratigraphic frameworks are crucial for unraveling the biocultural processes behind the dispersals of Homo sapiens across Europe. Detailed technological studies of lithic assemblages retrieved from multi-stratified sequences allow archaeologists to precisely model the chrono-cultural dynamics of the early Upper Paleolithic. However, it is of paramount importance to verify the integrity of these assemblages before building explanatory models of cultural change. In this study, multiple lines of evidence suggest that the stratigraphic sequence of Fumane Cave in northeastern Italy experienced minor post-depositional reworking, establishing it as a pivotal site for exploring the earliest stages of the Aurignacian. By conducting a systematic search for break connections between blade fragments and applying spatial analysis techniques, we identified three well-preserved areas of the excavation containing assemblages suitable for renewed archaeological investigations. Subsequent technological analyses, incorporating attribute analysis, reduction intensity, and multivariate statistics, have allowed us to discern the spatial organization of the site during the formation of the Protoaurignacian palimpsest A2–A1. Moreover, diachronic comparisons between three successive stratigraphic units prompted us to reject the hypothesis of techno-cultural continuity of the Protoaurignacian in northeastern Italy after the onset of the Heinrich Event 4. Based on the variability of the lithic and osseous artifacts, the most recent assemblage analyzed, D3b alpha, is now ascribed to the Early Aurignacian, aligning the evidence from Fumane with the current understanding of the development of the Aurignacian across Europe. Overall, this study demonstrates the high effectiveness of the break connection method when combined with detailed spatial analysis and lithic technology, providing a methodological tool particularly amenable to be applied to sites excavated in the past with varying degrees of recording accuracy.

The process by which Palaeolithic Europe was transformed from a Neanderthal-dominated region to one occupied exclusively by Homo sapiens has proven challenging to diagnose. A blurred chronology has made it difficult to determine when Neanderthals disappeared and whether modern humans overlapped with them. Italy is a crucial region because here we can identify not only Late Mousterian industries, assumed to be associated with Neanderthals, but also early Upper Palaeolithic industries linked with the appearance of early H. sapiens, such as the Uluzzian and the Aurignacian. Here, we present a chronometric dataset of 105 new determinations (74 radiocarbon and 31 luminescence ages) from four key southern Italian sites: Cavallo, Castelcivita, Cala, and Oscurusciuto. We built Bayesian-based chronometric models incorporating these results alongside the relative stratigraphic sequences at each site. The results suggest; 1) that the disappearance of Neanderthals probably pre-dated the appearance of early modern humans in the region and; 2) that there was a partial overlap in the chronology of the Uluzzian and Protoaurignacian, suggesting that these industries may have been produced by different human groups in Europe.